Large silverfish transplantation and proliferation technology

First, the broodstock fishing

The broodstock was selected to be spawned in the spawning season and spawning and breeding. The broodstock was caught by the ice sinking gill net and the broodstock should be collected within 2 hours from the capture of the broodstock to fertilization.

Second, dry artificial insemination

Select the mature broodstock, first squeezing the gonad of the female fish, and let the fish eggs flow into the dry fertilization vessel; then squeeze the crescent-shaped milky white testis near the male fish's vent hole so that the semen flows along the hip fins to the egg. After squeezing three male fish, the male and female anal fins and caudal fins were stirred to mix and fertilize the sperm and eggs. 20 ml of 0.7% physiological saline was added to activate the sperm, stirring was continued for 1 minute, and left for 1-2 minutes before being poured into the pelvic floor. in. When the fertilized eggs have a certain amount, rinse them several times with clean water and filter out the contaminants such as tissue blocks.

Third, incubation management

Select different hatching methods based on local conditions and the number of fertilized eggs.

1. Incubation of containers: Transfer the rinsed and clean fertilized eggs to the trays of fresh water, keep them at a depth of 2 cm, lay eggs at 500,000-1 million per square meter, stir for 2 hours, change the water once a day, and suck out dead eggs. .

2. Incubation pool Incubation: In the concrete floor, use brick or wood to build a rectangular hatching pond 1.5 meters wide and 3 meters long. Place a plastic sheet on the substrate and place 1 to 2 million grains of fertilized eggs per square meter. Water depth 4 cm, water temperature 2-5 °C, change the water once a day, each time the amount of water does not exceed 1/3.

3. Calculate the fertilization rate: In the hatching process, the embryonic development of the fertilized egg is often observed with a microscope or dissecting microscope. After the fertilized egg is developed to the middle of the midgut, the fertilization rate is calculated.

IV. Fertilized eggs

1. Location: Choose leeward sun, water body is stable, water depth is 1.5-2.5 meters, zooplankton is rich in bait, there are less enemy fish, and the sediment is cast in a hard or sandy place. The water ice ice eye specification is suitable for 1 meter 1.5 meters.

2. Transportation: Use nylon bags sealed oxygenation method to transport, 50,000-100,000 tablets per litre of water, water temperature during transport should be consistent with the temperature at the time of fertilization incubation.

3. Dispensing: Slowly immerse the egg bag in the water. When the water temperature in the bag is the same as the water temperature in the store, open the plastic bag and spread the eggs evenly in the water. Stir with a wooden board, wooden stick, etc. Avoid overlapping fertilized eggs.

V. Monitoring the growth of silver fish

After the fertilized eggs of giant whitebait are released, the growth, food habits, and gonadal development of large whitebait should be measured regularly, and the changes in water quality and feed should be regularly monitored to provide a scientific basis for the correct assessment of the amount of resources.

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