Nanjing Senbega stock, quality assurance, price concessions, kits preferred Senbega. This reagent is for research use only: This kit is used to determine the content of porcine cytomegalovirus antibody (CMV-Ab) in pig serum, plasma and related liquid samples. Experimental principle : The kit uses double antigen sandwich enzyme-linked immunosorbent assay (ELISA) to determine the porcine cytomegalovirus antibody (CMV-Ab) in the specimen. The microporous plate is coated with purified porcine adenovirus (ADV) antigen to prepare a solid phase antigen, which can be combined with the porcine cytomegalovirus antibody (CMV-Ab) in the sample, and washed to remove unbound antibody and other components. The antigen-antibody-enzyme-labeled antigen complex is then combined with the HRP-labeled antigen, and after thorough washing, the substrate TMB is added for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The absorbance (OD value) was measured at a wavelength of 450 nm using a microplate reader, and compared with the CUTOFF value to determine the presence or absence of a porcine cytomegalovirus antibody (CMV-Ab) in the specimen. Kit composition : Kit composition 48 hole configuration 96-well configuration save Instruction manual 1 copy 1 copy Sealing film 2 pieces (48) 2 pieces (96) sealed bag 1 1 Enzyme label coated plate 1×48 1×96 Store at 2-8 ° C Negative control 0.5ml × 1 bottle 0.5ml × 1 bottle Store at 2-8 ° C Positive control 0.5ml × 1 bottle 0.5ml × 1 bottle Store at 2-8 ° C Enzyme standard reagent 3 ml × 1 bottle 6 ml × 1 bottle Store at 2-8 ° C Sample diluent 3 ml × 1 bottle 6 ml × 1 bottle Store at 2-8 ° C Developer A solution 3 ml × 1 bottle 6 ml × 1 bottle Store at 2-8 ° C Developer B solution 3 ml × 1 bottle 6 ml × 1 bottle Store at 2-8 ° C Stop solution 3ml × 1 bottle 6ml × 1 bottle Store at 2-8 ° C Concentrated washing solution (20ml × 20 times) × 1 bottle (20ml × 30 times) × 1 bottle Store at 2-8 ° C Sample processing and requirements : 1. Serum: The blood is naturally coagulated for 10-20 minutes at room temperature and centrifuged for about 20 minutes (2000-3000 rpm). The supernatant is carefully collected, and if precipitation occurs during storage, it should be centrifuged again. 2. Plasma: EDTA or sodium citrate should be selected as an anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm). The supernatant is carefully collected, and if a precipitate forms during storage, it should be centrifuged again. 3. Urine: Collect with a sterile tube and centrifuge for about 20 minutes (2000-3000 rpm). The supernatant is carefully collected, and if a precipitate forms during storage, it should be centrifuged again. The chest and ascites and cerebrospinal fluid are referred to. 4. Cell culture supernatant: When detecting secreted components, collect them in a sterile tube. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. When the components in the cells were detected, the cell suspension was diluted with PBS (pH 7.2-7.4), and the cell concentration reached about 1 million/ml. By repeated freezing and thawing, the cells are destroyed and the intracellular components are released. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again. 5. Tissue specimen: After cutting the specimen, weigh the weight. Add a certain amount of PBS, pH 7.4. It was quickly frozen and stored in liquid nitrogen for use. The specimen still maintains a temperature of 2-8 ° C after melting. A certain amount of PBS (pH 7.4) was added, and the specimen was homogenized by hand or homogenizer. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. One part of the package is to be tested, and the rest is frozen for use. 6. The specimens should be extracted as soon as possible after collection, and the extraction should be carried out according to the relevant literature. The experiment should be carried out as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 °C, but repeated freezing and thawing should be avoided. 7. Samples containing NaN3 cannot be detected because NaN3 inhibits horseradish peroxidase (HRP) activity. Operation steps : The result is judged: Test validity: mean value of positive control well ≥ 1.00; mean value of negative control ≤ 0.10 CUT OFF calculation: critical value = negative control well average + 0.15 Negative judgment: sample OD value < critical value (CUT OFF) is negative for porcine cytomegalovirus antibody (CMV-Ab) Positive judgment: sample OD value ≥ critical value (CUT OFF) is positive for porcine cytomegalovirus antibody (CMV-Ab) Precautions 1. The operation is carried out in strict accordance with the instructions. The components of the different batches of this reagent shall not be mixed. 2. The kit should be taken out from the refrigerated environment and should be used at room temperature for 15-30 minutes before use. If the enzyme label is unsealed after opening, the slats should be stored in a sealed bag. 3. Concentrated washing liquid may crystallize out. When diluted, it can be heated and dissolved in a water bath. The washing will not affect the result. 5. Please keep the substrate away from light. 6. The test results must be determined by the microplate reader. When using dual-wavelength detection, the reference wavelength is 630nm. 7. All samples, washings and various wastes should be treated as infectious materials. The stop solution is 2M sulfuric acid and must be used safely. Storage conditions and expiration date 1. The kit is stored at: 2-8 °C. 2. 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